Gage Lab

Dept. of Molecular and Cell Biology
University of Connecticut - Storrs

Publications

•S. J. Taerum, J. Micciulla, G. Corso, B. Steven, D. J. Gage and L. R. Triplett (2021). 18S rRNA gene amplicon sequencing combined with culture-based surveys of maize rhizosphere protists reveal dominant, plant-enriched and culturable community members. Environmental Microbiology Reports.
https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.13038  

•Christopher J. Hawxhurst, Jamie L. Micciulla, Charles M. Bridges, Leslie M. Shor and Daniel J. Gage (2021). Soil protists can actively redistribute beneficial bacteria along Medicago truncatula roots. Biorxiv: https://www.biorxiv.org/content/10.1101/2021.06.16.448774v1

•Bridges, C.M.  and Daniel. J. Gage (2021). Draft Genome Sequences of Dysgonomonas sp. Strains GY75and GY617, Isolated from the Hindgut of Reticulitermes flavipes. Microbiol. Resour. Announc. 10:e00079-21
https://mra.asm.org/content/10/12/e00079-21

•Bridges, C.M., Nelson, M.C., Graf, J. and Daniel. J. Gage (2021). Draft Genome Sequences of Dysgonomonas sp. Strains BGC7and HGC4, Isolated from the Hindgut of a Lower Termite. Microbiol. Resour. Announc. 10:e01427-20.
https://doi.org/10.1128/MRA.01427-20

• Bridges, C.M. and Daniel. J. Gage (2021). Development and application of aerobic, chemically defined media for Dysgonomonas. Anaerobe 67:102302. https://doi.org/10.1016/j.anaerobe.2020.102302

•Pirhanov, A.; Bridges, CM; Goodwin, RA; Gao, Y; Furrer, J; Shor, LM; Gage, DJ and Cho, YK. (2021) Optogenetics in Sinorhizobium meliloti enables spatial control of exopolysaccharide production and biofilm structure. ACS Synthetic Biology:  https://pubs.acs.org/doi/pdf/10.1021/acssynbio.0c00498

•Taerum, SJ; Steven, B; Gage, DJ and Triplett, LR. (2020) Validation of a PNA clamping method for reducing host DNA amplification and increasing eukaryotic diversity in rhizosphere microbiome studies. Phytobiomes 4:291-302.  get pdf

•M. E. Stephens and D. J. Gage. (2018) Oligotyping bacterial symbionts of hindgut protists reveals complex population structures and transmission trends in the termite Reticulitermes flavipes. PLoS ONE 15(5): e0233065. https://doi.org/10.1371/journal.pone.0233065. get pdf

•Guo Y-S, Furrer JM, Kadilak AL, Hinestroza HF, Gage DJ, Cho YK and Shor LM (2018). Bacterial extracellular polymeric substances amplify water content variability at the pore scale. Front. Environ. Sci. 6:93. doi: 10.3389/fenvs.2018.00093 get pdf

•B. C. Cruz, J. M. Furrer, D Doughert, D. J. Gage, Y. K. Cho, L. M. Shor (2018). Pore-scale water dynamics during drying and the impacts of structure and surface wettability. Water Resources Research 53:5585-5600. get pdf

•Deng, J., Orner, E. P., Chau, J. F., Anderson, E. M., Kadilak, A.L., Rubinstein, R. L., Bouchillon, G. M., Goodwin, R. A., Gage, D. J. and L. M. Shor. (2015). Synergistic effects of soil microstructure and bacterial EPS on drying rate in emulated soil micromodels. Soil Biology Biochem. 83:116-124 get pdf

•Rubinstein, R., Kadilak, A., Cousens, V., Gage, D. J. and L. Shor (2015). Protist-facilitated particle transport using emulated soil micromodels. Environ. Sci. Tech. Environ. Sci. Technol. 49:1384-1391. get pdf

•Goodwin, R. A. and D. J. Gage. (2014) Biochemical characterization of a nitrogen-type phosphotransferase system reveals enzyme EINtr integrates carbon and nitrogen signaling in Sinorhizobium meliloti. J. Bacteriol. 196:1901 get pdf

•Sheftic, S.R., White, E., Gage, D. & Alexandrescu, A.T. (2014). NMR structure of the HWE kinase associated response regulator Sma0114 in its activated state. Biochemistry 53: 311-22. get pdf

•Herron, P. M., Gage, D. J., Arango-Pinedo, C., Haider, Z. K., and Z. G. Cardon (2013). Better to light a candle than curse the darkness: illuminating spatial localization and temporal dynamics of rapid microbial growth in the rhizosphere. Frontiers in Plant Sci. Article 323. doi:10.3389/fpls.2013.00323. get pdf

•Sheftic, S. R., Garcia, P. P., White, E., Robinison, V. L., Gage, D. J. and A. T. Alexandrescu (2012) Nuclear magnetic resonance structure and dynamics of the response regulator Sma0114 from Sinorhizobium meliloti. Biochemistry 51: 6932. get pdf

•Garcia, P. P. Bringhurst, R.M. and D. J. Gage (2010). Characterization of a two-component regulatory system which regulates succinate-mediated catabolite repression in Sinorhizobium meliloti. J. Bacteriol 192: 5725-5735. get pdf

• Sheftic, S. R., Garcia, P. P., Robinson, V. L., Gage, D. J. and A. T Alexandrescu (2010) NMR assignments for the Sinorhizobium meliloti response regulator Sma0114. Biomolecular NMR Assignments 5:55-8 get pdf

•P. M. Herron, D. J. Gage, and Z. G. Cardon (2010) Microscale water potential gradients visualized in soil around plant root tips using microbiosensors. Plant, Cell and Environment 33:199-210. get pdf

•Arango-Pinedo and D. J. Gage. (2009) Plasmids that insert into the rhamnose utilization locus, rha: a versatile tool for genetic studies in Sinorhizobium meliloti. Journal of Molecular Biology and Biotechnology 17:201-210 get pdf

•C. Arango-Pinedo and D. J. Gage (2009) HPrK regulates succinate-mediated catabolite repression in the Gram negative symbiont Sinorhizobium meliloti. J. Bacteriol. 191:298-309 get pdf

•Gage, D. J., Herron, P. M., Arango-Pinedo, C., and Z. G. Cardon (2008). Live reports from the soil grain--the promise and challenge of microbiosensors. Functional Ecology 22:983. get pdf

•Arango-Pinedo, C., Bringhurst, R.M. and D. J. Gage (2008). Sinorhizobium meliloti mutants lacking phosphotransferase system enzyme HPr or EIIA are altered in diverse processes, including carbon metabolism, cobalt requirements, and succinoglycan production. J. Bacteriol. 190:2947 get pdf

•Z. Cardon and D. J. Gage (2006) Resource exchange in the rhizosphere – molecular tools and the microbial perspective. Ann. Rev. Ecology, Evolution and Systematics 37: 459-488 get pdf

•H. Monahan, C. Arango-Pinedo and D. J. Gage (2006) Architecture of infection thread networks in developing root nodules. Plant Physiology 140: 661-670 get pdf

•D. J. Gage (2004) Infection and invasion of roots by symbiotic, nitrogen-fixing, rhizobia during nodulation  of temperate legumes. Microbiol. Mol. Biol. Rev. get pdf 

•M. Rosado and D. J. Gage. (2003) Transcriptional control of a rRNA promoter of the nodulating symbiont Sinorhizobium meliloti. FEMS Microbiol. Letters get pdf

•D. J. Gage (2002) Analysis of infection thread development after coinoculation of alfalfa with Gfp-and Dsred-expressing Sinorhizobium meliloti J. Bact. get pdf

•Bringhurst, R. M. and D. J. Gage (2002) Inducer exclusion/expulsion plays a key role in succinate-mediated catabolite repression in Sinorhizobium meliloti. J. Bacteriology 184:5385-5392 get pdf

•Bringhurst, R. M., Cardon, Z., and D. J. Gage (2001). Galactosides in the rhizosphere: utilization by Sinorhizobium meliloti and development of a biosensor. Proc. Nat. Acad. Sci. 98:4540-4545 get pdf

•Gage, D. J., and W. Margolin. (2000). Hanging by a thread: invasion of legume plants by rhizobia. Current Opinion in Microbiology 3:613-617 get pdf

•Bringhurst, R. M., and D. J. Gage (2000) An AraC-like transcriptional activator is required for induction of genes needed for b-galactoside utilization in Sinorhizobium meliloti. FEMS Microbiol. Letters 188:23-27 get pdf

•Gage, D. J., and S. R. Long. (1998) -galactoside uptake in Rhizobium meliloti: isolation and characterization of agpA, a gene encoding a periplasmic binding protein required for melibiose and raffinose utilization. J. Bacteriology 180: 5739 - 5748 get pdf

•Gage, D. J., Bobo, T. and S. R. Long. (1996). Use of green fluorescent protein to visualize the early events of symbiosis between Rhizobium meliloti and alfalfa, Medicago sativa. J. Bacteriology 178:7159-7166 get pdf

•Gage, D.J., and F.C. Neidhardt. (1993). Adaptation of Escherichia coli to an uncoupler of oxidative phosphorylation, 2,4-dinitrophenol. J. Bact. get pdf

•Gage, D.J., and F.C. Neidhardt. (1993). Modulation of the heat shock response by one-carbon metabolism in Escherichia coli. J. Bacteriology 175: 1961. get pdf

•Faustman, E.M., Z. Kirby, D. Gage, and M. Varnum, (1989). In vitro developmental toxicity of five direct-acting alkylating agents in rodent embryos: structure-activity patterns. Teratology 40: 199

Book Chapters
•Gage, D. J. (2009) Legume Nodule Formation. In D. W. Emerich and H. B. Krishnan (ed.), Nitrogen Fixation in Crop Production. American Society of Agronomy, Madison, WI, USA.

•Gage, D. J. (2009) Architecture of infection thread networks in nitrogen-fixing root nodules. In Plant Cell Monographs Volume 12, Root Hairs, A. M. C. Emons and T. Ketelaar (ed.), Springer, Heidelberg, Germany.

Other Publications
• J. Deng, D. J. Gage and L. M. Shor (2014). Variations in moisture retention of contact-printed soil bacteria surface colonies using confocal microscopy. New England Bioengineering Conference Proceedings, Boston

•Gage, D. J. (2008) Review of “Molecular Genetics of Bacteria, 3rd edition by Larry Snyder and Wendy Champness”, Quarterly Rev. Biology 83:115-116